【學(xué)術(shù)報告】Developing?Novel?Nucleic?Acid-based?Tools?for?on-site?Fluoride?Detection,?and?Spatial?Imaging?of?GlycoRNAs
日期：2024-06-11
閱讀：3926
主題：Developing?Novel?Nucleic?Acid-based?Tools?for?on-site?Fluoride?Detection,?and?Spatial?Imaging?of?GlycoRNAs
報告人：Professor?Yuan?Ma
時間：2024年6月11日（周二）14：00
地點：徐名材報告廳（霞光樓200）
邀請人：朱新遠(yuǎn) 教授 / 顏德岳 教授
個人簡介
Yuan?Ma?joined?the?Chemistry?faculty?at?Rice?in?2024?as?Kenneth?S.?Pitzer-Schlumberger?Junior?Faculty?Chair?and?Norman?Hackerman-Welch?Young?Investigator.?Before?this?appointment,?Yuan’s?postdoctoral?research?was?conducted?at?the?University?of?Illinois?at?Urbana-Champaign?and?the?University?of?Texas?at?Austin?in?the?Department?of?Chemistry,?under?the?guidance?of?Professor?Yi?Lu.?Her?postdoctoral?research?focused?on?developing?bioimaging?techniques?to?study?glycoRNAs?and?metabolites,?screening?novel?nucleic?acid?aptamers,?and?designing?biosensors?for?on-site?detection.?Yuan?completed?her?Ph.D.?at?Shanghai?Jiao?Tong?University,?co-advised?by?Professors?Deyue?Yan,?Xinyuan?Zhu,?and?Chuan?Zhang.?Her?doctoral?research?was?centered?on?developing?innovative?nanomedicine?technologies?for?biomedical?applications,?and?DNA?nanotechnology.?Yuan?received?her?B.A.?in?Materials?Physics?from?Nanjing?University?advised?by?Professor?Jin?Zhu.?She?has?been?selected?as?Provost?Early?Career?Fellow?at?the?University?of?Texas?at?Austin?and?the?Leading?Edge?Fellow.
講座摘要
Nucleic?acids?have?emerged?as?powerful?and?functional?materials?due?to?their?ability?to?achieve?precise?hybridization?between?nucleic?acids?and?molecular?recognition?toward?a?wide?range?of?targets.?Despite?the?promise,?their?applications?are?still?limited.?In?this?seminar,?I?will?present?two?examples?that?demonstrate?innovations?in?experimental?designs?have?overcome?the?limitations?and?resulted?in?wider?applications.?First,?utilizing?the?property?of?precise?molecular?recognition,?I?have?designed?a?riboswitch?sensor?for?on-site?and?real-time?detection?of?fluoride.?Specifically,?current?portable?fluoride?sensors?are?limited?by?their?poor?performance?in?aqueous?solutions,?such?as?low?selectivity,?sensitivity,?and?narrow?dynamic?range.?To?overcome?these?limitations,?I?coupled?a?fluoride?riboswitch-regulated?transcription?with?CRISPR-Cas13-based?signal?amplification,?allowing?quantitative?detection?of?fluoride?in?aqueous?solutions?with?high?sensitivity,?high?selectivity,?and?a?wide?dynamic?range.?This?work?has?also?expanded?the?application?of?nucleic?acid?sensors?from?cations?and?neutral?molecules?to?anions.?Second,?utilizing?both?properties?of?precise?hybridization?and?molecular?recognition,?I?have?developed?a?nucleic?acid-based?bioimaging?tool?to?investigate?glycoRNA?biology.?While?glycosylated?RNAs?(glycoRNAs)?have?recently?been?discovered?as?a?new?class?of?glycosylated?biomolecules,?our?understanding?of?their?roles?is?limited?because?of?a?lack?of?visualization?methods.?To?overcome?this?limitation,?I?developed?the?first?spatial?imaging?method?to?visualize?glycoRNAs?in?cells?with?high?sensitivity?and?selectivity.?Using?this?method,?we?discovered?spatial?distributions?of?glycoRNAs?on?lipid?rafts?and?cell?trafficking?through?SNARE?protein-mediated?secretory?exocytosis.?We?found?that?surface?glycoRNAs?are?inversely?associated?with?tumor?malignancy?and?metastasis?and?that?glycoRNAs?may?mediate?cell-cell?interactions?during?the?immune?response.?This?work?has?expanded?the?toolbox?for?glycoRNA?research,?paving?the?way?for?exploring?the?roles?of?glycoRNAs?in?diverse?biological?processes.?Together,?the?two?examples?demonstrate?that?innovations?in?developing?nucleic?acid-based?tools?can?result?in??better?sensors?for?anions,?and?a?deeper?understanding?of?glycoRNAs?in?biology?and?diseases.
