【學(xué)術(shù)報(bào)告】Engineering?Modified?Nucleic?Acids?for?Bioimaging?Applications
日期：2024-05-29
閱讀：345
主題：Engineering?Modified?Nucleic?Acids?for?Bioimaging?Applications
報(bào)告人:Professor?Quanbing?Mou
時(shí)間：2024年6月11日（周二）15：00
地點(diǎn)：徐名材報(bào)告廳（霞光樓200）
邀請(qǐng)人:朱新遠(yuǎn) 教授
個(gè)人簡(jiǎn)介
Dr.?Quanbing?Mou?is?an?Assistant?Professor,?Norman?Hackerman-Welch?Young?Investigator,?and?Kenneth?S.?Pitzer-Schlumberger?Junior?Faculty?Chair?in?the?Department?of?Chemistry?at?Rice?University.?He?earned?his?B.E.?degree?in?Polymer?Science?and?Engineering?from?Sichuan?University,?where?he?conducted?research?in?Prof.?Changsheng?Zhao’s?group?on?the?synthesis?of?sulfonated?polyethersulfone?membranes?for?blood?purification.?In?2012,?he?enrolled?in?the?Ph.D.?program?at?Shanghai?Jiao?Tong?University.?His?thesis?work,?supervised?by?Profs.?Deyue?Yan,?Xinyuan?Zhu,?and?Chuan?Zhang,?focused?on?nucleoside?analog-containing?precise?nanodrugs?for?cancer?therapy.?In?2018,?Dr.?Mou?joined?Prof.?Yi?Lu's?laboratory?at?the?University?of?Illinois?Urbana-Champaign?as?a?postdoctoral?researcher.?There,?he?concentrated?on?engineering?DNAzyme-based?biosensors?for?metal?ion?detection?and?developing?in-situ?biosensors?to?image?metabolites?and?bioproducts?in?plants.?In?2021,?he?moved?to?the?University?of?Texas?at?Austin?with?Prof.?Yi?Lu,?where?he?explored?new?research?areas,?including?engineering?CRISPR/Cas?systems?for?biosensing?and?biomedical?applications,?developing?a?super-resolution?imaging?technique?called?DNAzyme-PAINT?for?metal?ion?detection?in?living?systems,?and?creating?innovative?caging?strategies?for?DNAzyme-based?biosensing.?In?his?independent?lab,?Dr.?Mou?focuses?on?building?various?chemical?biology?tools?to?understand?RNA?biology?and?promote?the?applications?of?RNA-related?technologies.
報(bào)告摘要
Nucleic?acids?are?essential?not?only?as?carriers?of?genetic?information?but?also?as?versatile?functional?materials.?Modifications?of?nucleic?acids?play?a?crucial?role?in?transmitting?genetic?information?and?enhancing?the?effectiveness?as?functional?materials.?In?this?seminar,?I?will?discuss?two?applications?of?engineered?modified?nucleic?acids?in?bioimaging.
Firstly,?I?will?discuss?how?nucleic?acid?modifications?can?drastically?improve?the?delivery?efficiency?of?nucleic?acids?in?plant?cells?for?glucose?bioimaging.?Plants?are?promising?renewable?resources?for?biofuel?production,?which?still?face?the?challenge?of?developing?new?plant?species?with?high?productivity?in?energy?molecules,?such?as?glucose.?Therefore,?there?is?an?urgent?need?to?develop?in?situ?glucose?bioimaging?tools?to?guide?plant?engineering.?Traditional?glucose?aptamer-based?sensors?face?delivery?challenges?in?intact?plants.?To?overcome?this?challenge,?I?have?developed?a?thiol-mediated?uptake?method?that?efficiently?introduces?nucleic?acids?into?plant?cells.?This?method?allows?for?effective?in?situ?imaging?of?glucose?in?plants,?which?serves?as?a?new?platform?to?guide?the?plant?engineering?for?biofuel?production.?
Secondly,?I?will?introduce?the?development?of?super-resolution?imaging?tools?for?3D?Mg2+?imaging?in?single?cells.?Mg2+?is?related?to?various?diseases,?including?diabetes,?Parkinson’s?disease,?and?hypertension.?However,?how?Mg2+?is?regulated?in?single?cells?remains?largely?unexplored.?To?bridge?this?gap,?I?have?created?a?novel?super-resolution?bioimaging?tool?for?Mg2+,?DNAzyme-PAINT,?which?incorporates?modifications?like?disulfide?repeats,?photocage-dG,?and?locked?nucleic?acids.?This?tool?has?enabled?the?first?time?3D?super-resolution?imaging?of?free?Mg2+?in?single?cells,?revealing?a?strong?association?between?Mg2+?and?mitochondria.?This?methodology?can?be?generalized?to?study?other?metal?ions?by?altering?the?DNAzyme.
